Click image to see more details
Product Info Summary
| SKU: | M03628-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-LMAN1 Monoclonal Antibody
SKU/Catalog Number
M03628-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-LMAN1 Monoclonal Antibody catalog # M03628-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LMAN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03628-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEBI-12
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human LMAN1 Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03628-1 is reactive to LMAN1 in Human, Mouse, Rat
Applications
M03628-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
66 kDa
Calculated molecular weight
57.549kDa
Background of LMAN1
Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of LMAN1 expression in (1) HeLa cell lysate; (2) Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For LMAN1 (Source: Uniprot.org, NCBI)
Gene Name
LMAN1
Full Name
Protein ERGIC-53
Weight
57.549kDa
Alternative Names
ERGIC-53; ERGIC53ER-Golgi intermediate compartment 53 kDa protein; F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53; FMFD1; Gp58; intracellular mannose specific lectin; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1; lectin, mannose-binding, 1; MCFD1coagulation factor V-factor VIII combined deficiency; MR60; protein ERGIC-53 LMAN1 ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58 lectin, mannose binding 1 protein ERGIC-53|ER-Golgi intermediate compartment 53 kDa protein|endoplasmic reticulum-golgi intermediate compartment protein 53|intracellular mannose-specific lectin MR60
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LMAN1, check out the LMAN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LMAN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-LMAN1 Monoclonal Antibody (M03628-1)
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No publications found for M03628-1
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Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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- Bioscience Research Protocol Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-LMAN1 Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary cancer using anti-LMAN1 Monoclonal antibody M03628-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
I see that the anti-LMAN1 Monoclonal antibody M03628-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-23
Question
Is this M03628-1 anti-LMAN1 Monoclonal antibody reactive to the isotypes of LMAN1?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
The immunogen of M03628-1 anti-LMAN1 Monoclonal antibody is A synthesized peptide derived from human LMAN1 Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-11
Question
Will anti-LMAN1 Monoclonal antibody M03628-1 work for WB with mammary cancer?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
According to the expression profile of mammary cancer, LMAN1 is highly expressed in mammary cancer. So, it is likely that anti-LMAN1 Monoclonal antibody M03628-1 will work for WB with mammary cancer.
Boster Scientific Support
Answered: 2019-10-08
Question
I was wanting to use your anti-LMAN1 Monoclonal antibody for WB for human mammary cancer on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mammary cancer identification?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
You can see on the product datasheet, M03628-1 anti-LMAN1 Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human mammary cancer in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-18
Question
Our lab want to know about to test anti-LMAN1 Monoclonal antibody M03628-1 on human mammary cancer for research purposes, then I may be interested in using anti-LMAN1 Monoclonal antibody M03628-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
The products we sell, including anti-LMAN1 Monoclonal antibody M03628-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-04
Question
Do you have a BSA free version of anti-LMAN1 Monoclonal antibody M03628-1 available?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-LMAN1 Monoclonal antibody M03628-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-27
